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This intensive laboratory and computer-based training course introduces participants to genome-wide analysis of gene function, including hands-on experience in performing RNAi, expression profiling using different platforms and yeast 2-hybrid analyses. Practical demonstrations of the latest Solexa high throughput sequencing techniques applied on cDNA sequencing will also be included in the programme. Laboratory work will be complemented by training in state-of-the-art approaches to data analysis and interpretation through the use of a range of bioinformatics and systems biology tools, including R, Bioconductor, GeneSpring, network analysis and pathway modelling. Particular emphasis will be placed upon the integration of complementary approaches to ask specific biological questions. The course will include seminars by distinguished international speakers who will present cutting-edge research in functional genomics and systems biology. Programme RNAi RNAi has recently emerged as one of the most powerful tools for examining gene function. Different RNAi technologies will be compared both for RNAi in mammalian cells in culture and in C. elegans in vivo. Participants will carry out a high-throughput RNAi screen, and this will illustrate many of the theoretical considerations in designing such large-scale RNAi experiments. We will also illustrate how RNAi can be combined with microarray analysis to identify pathway targets. Expression profiling Practical guidance will be given in the use of commercial (e.g. Affymetrix and Illumina) and spotted oligo microarray platforms for the analysis of gene expression including miRNAs.This will involve the use of different RNA and miRNA labelling strategies, hybridisation and image analysis.Participants will be given training in aspects of microarray data analysis and interpretation using a range of commercial and academic software tools. Practical instruction will also include microarray and RNAi data validation by quantitative PCR methods.Finally, there will be the opportunity to discuss the theoretical considerations for microarray fabrication, experimental design and the relative merits of different expression profiling platforms, including emerging technologies. Yeast two-hybrid analysis Yeast two-hybrid analysis will be used as an approach to identifying novel protein-protein interactions. Experiments will include the construction of baits, library screening, and analysis of clones to minimise artefacts. Bioinformatics This will provide an overview and discussion of current bioinformatics resources most relevant to functional genomics investigations.These will include microarray data storage and standards, genome browsers, gene/protein annotation and pathway analysis. Systems biology The course will cover integration of data into interaction and regulatory networks and systems biology approaches to mining and modelling of these data. Informal tutorials from academic groups and commercial organisations will complement the laboratory sessions. Course instructors Andrew Fraser (The Wellcome Trust Sanger Institute, Hinxton, UK) Tom Freeman (Division of Pathway Medicine, Edinburgh, UK) David Markie (Dunedin School of Medicine, New Zealand) Ioannis Ragoussis (Wellcome Trust Centre for Human Genetics, Oxford, UK) Anton Enright (European Bioinformatics Institute, Hinxton, UK) Guest speakers Eran Segal (Weizmann Institute of Science, Israel) Rickard Sandberg (Karolinska Institute, Sweden) Chris Ponting (MRC Functional Genomics Unit, Oxford, UK) Cost This is a residential course, without exception, and there is a fee of ?975 towards board and lodging for academic applicants. The fee for commercial (non-academic) applicants is ?5725. The course tuition fees are subsidised by The Wellcome Trust for scientists based in academic institutions from anywhere in the world. Bursaries Limited bursaries are available for academics (50 per cent of fee) and are subject to open competition. Applications Applica

This intensive laboratory and computer-based training course introduces participants to genome-wide analysis of gene function, including hands-on experience in performing RNAi, expression profiling using different platforms and yeast 2-hybrid analyses. Practical demonstrations of the latest Solexa high throughput sequencing techniques applied on cDNA sequencing will also be included in the...

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